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pEASY 系列克隆载体
  • CT501-01.JPG
CT501-01.JPG

pEASY®-T5 Zero Cloning Kit


产品介绍

该载体通过自杀基因表达与否筛选阳性重组子。当载体与片段连接成功时,自杀基因无法正确表达,包含重组子的细胞可以正常生长;当载体与片段连接不成功时,自杀基因正确表达,包含载体的细胞无法生长,即“Zero”背景。适用于TA克隆。


产品组成

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References

Xia B , Han D , Lu X , et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale[J]. Nature Methods, 2015, 12(11):1047-50.
Gao Y , Chen J , Li K , et al. Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming[J]. Cell Stem Cell, 2013, 12(4):453-469.
Le R , Kou Z , Jiang Y , et al. Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells[J]. Cell Stem Cell, 2014, 14(1):27-39.
Gao S , Zheng C , Chang G , et al. Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells[J]. Nature Communications, 2015, 6:6318.
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells[J]. Cell Research, 2014, 24(3):293-306.

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